Natural and Applied Science Institute, Department of Biology, Çukurova University, Adana, Turkey.
Drug Chem Toxicol. 2012 Jan;35(1):11-9. doi: 10.3109/01480545.2011.564181. Epub 2011 Jul 21.
The genotoxicity of tannic acid (TA, tannin) were investigated using chromosome aberration (CA), sister chromatid exchange (SCE), and micronucleus (MN) test systems in human peripheral lymphocytes. Also, the antigenotoxicity of TA against known mutagen EMS was also examined. The lymphocytes were treated with 1.74 × 10(-5), 3.49 × 10(-5), and 6.98 × 10(-5) µM of TA for 24- and 48-hour treatment periods. For the antigenotoxicity of TA, the lymphocytes were treated with three different concentrations of TA and 2.71 µM of EMS. TA synergically induced the CA alone and with the mixture of EMS. However, TA did not induce the SCE alone, whereas TA and EMS as a mixture also synergically induced SCE. TA alone showed no clear effect on micronucleus formation, and it did not induce the MN when used with EMS as a mixture. In addition, TA showed a synergistic cytotoxic effect by decreasing the mitotic and nuclear division indices. The replication index was decreased at all concentrations for 48 hours of treatment time by TA and EMS as a mixture.
采用染色体畸变（CA）、姐妹染色单体交换（SCE）和微核（MN）试验系统，研究了鞣酸（TA，单宁）的遗传毒性。同时，还研究了 TA 对已知诱变剂 EMS 的抗原毒性。用 1.74×10（-5）、3.49×10（-5）和 6.98×10（-5）µM 的 TA 处理人外周血淋巴细胞 24 和 48 小时。对于 TA 的抗原毒性，用三种不同浓度的 TA 和 2.71µM 的 EMS 处理淋巴细胞。TA 单独和与 EMS 混合物协同诱导 CA。然而，TA 单独不诱导 SCE，而 TA 和 EMS 混合物也协同诱导 SCE。TA 单独对微核形成没有明显影响，与 EMS 混合使用也不诱导 MN。此外，TA 通过降低有丝分裂和核分裂指数表现出协同的细胞毒性作用。TA 和 EMS 混合物在所有浓度下均在 48 小时处理时间内降低了复制指数。
