Celikler Serap, Vatan Ozgur, Yildiz Gamze, Bilaloglu Rahmi
Biology Department, Science Arts Faculty, Uludag University, Bursa, Turkey.
Food Chem Toxicol. 2009 Apr;47(4):796-801. doi: 10.1016/j.fct.2009.01.010. Epub 2009 Jan 12.
The genome is constantly exposed to agents, both exogenous and endogenous, that damage DNA. Consequently, it is very important that determination of this agents and the protective agents. In this work, we evaluated the antigenotoxic/antimutagenic activity of the crude ethanolic extracts of Codium tomentosum Stackhouse (Chlorophyceae) (CTE), collected from The Coast of South East Marmara Sea, in human lymphocytes culture in vitro against genotoxic/mutagenic agents MMC, EMS and H(2)O(2) by using chromosome aberration (CA), sister chromatid exchange (SCE) and micronuclei (MN) assays as experimental endpoints. Also, in the present study, we determined total phenolic content and total antioxidant capacity (in soluble lipid and water). In addition, total protein, total carbohydrate, vitamins (A, C and E) and pigments (chlorophyll a, chlorophyll b and carotene) contents were also determined. Results of CA, SCE and MN tests show that CTEs have not shown genotoxic effect. In CTE plus MMC-, EMS- or H(2)O(2)- treated cultures, CA, SCE and MN frequency which induced by MMC, EMS or H(2)O(2) has been decreased significantly (p<0.05-0.001). This is the first report on genotoxicity/antigenotoxicity and anti-oxidative capacity of Codium tomentosum. Our results have clearly shown that CTE has strong anti-oxidative and antigenotoxic effect.
基因组不断受到外源性和内源性因素的影响，这些因素会损害DNA。因此，确定这些因素以及保护因素非常重要。在这项工作中，我们评估了从马尔马拉海东南部海岸采集的绒毛仙菜（绿藻纲）粗乙醇提取物（CTE）在体外人淋巴细胞培养中对遗传毒性/诱变剂丝裂霉素C（MMC）、甲基磺酸乙酯（EMS）和过氧化氢（H₂O₂）的抗遗传毒性/抗诱变活性，采用染色体畸变（CA）、姐妹染色单体交换（SCE）和微核（MN）试验作为实验终点。此外，在本研究中，我们测定了总酚含量和总抗氧化能力（在可溶性脂质和水中）。另外，还测定了总蛋白、总碳水化合物、维生素（A、C和E）和色素（叶绿素a、叶绿素b和胡萝卜素）的含量。CA、SCE和MN试验结果表明，CTE未显示出遗传毒性作用。在CTE加MMC、EMS或H₂O₂处理的培养物中，由MMC、EMS或H₂O₂诱导的CA、SCE和MN频率显著降低（p<0.05 - 0.001）。这是关于绒毛仙菜遗传毒性/抗遗传毒性和抗氧化能力的首次报告。我们的结果清楚地表明，CTE具有很强的抗氧化和抗遗传毒性作用。
