Key Laboratory of Jiangsu Preventive Veterinary Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, People's Republic of China.
Virology. 2011 Sep 1;417(2):379-84. doi: 10.1016/j.virol.2011.06.021. Epub 2011 Jul 19.
The HA protein of the 2009 pandemic H1N1 viruses (H1N1pdm) is antigenically closely related to the HA of classical North American swine H1N1 influenza viruses (cH1N1). Since 1998, through mutation and reassortment of HA genes from human H3N2 and H1N1 influenza viruses, swine influenza strains are undergoing substantial antigenic drift and shift. In this report we describe the development of a novel monoclonal antibody (S-OIV-3B2) that shows high hemagglutination inhibition (HI) and neutralization titers not only against H1N1pdm, but also against representatives of the α, β, and γ clusters of swine-lineage H1 influenza viruses. Mice that received a single intranasal dose of S-OIV-3B2 were protected against lethal challenge with either H1N1pdm or cH1N1 virus. These studies highlight the potential use of S-OIV-3B2 as effective intranasal prophylactic or therapeutic antiviral treatment for swine-lineage H1 influenza virus infections.
2009 年大流行的 H1N1 病毒（H1N1pdm）的 HA 蛋白在抗原性上与经典的北美猪源 H1N1 流感病毒（cH1N1）密切相关。自 1998 年以来，通过人类 H3N2 和 H1N1 流感病毒的 HA 基因的突变和重配，猪流感株正在经历大量的抗原漂移和转变。在本报告中，我们描述了一种新型单克隆抗体（S-OIV-3B2）的开发，该抗体不仅对 H1N1pdm，而且对α、β和γ簇的猪源 H1 流感病毒代表株具有高血凝抑制（HI）和中和效价。单次鼻腔内给予 S-OIV-3B2 的小鼠可免受 H1N1pdm 或 cH1N1 病毒的致死性攻击。这些研究强调了 S-OIV-3B2 作为预防或治疗猪源 H1 流感病毒感染的有效鼻腔内预防性或治疗性抗病毒治疗药物的潜在用途。
