Park Yong Ho, Kim Sun Gui, Lee Jin Woo, Yoon Young Hoon
Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Insititute for Brain Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea.
Int J Pediatr Otorhinolaryngol. 2011 Sep;75(9):1176-80. doi: 10.1016/j.ijporl.2011.06.015. Epub 2011 Jul 19.
The purpose of this study was to evaluate the applicability of different calcium phosphate types after mastoid obliteration procedure using the guinea pig as an animal model.
Twenty six male guinea pigs were used for the experimental study. Four guinea pigs were used as a normal control group, with the remaining used for the experimental group. After bulla mucosa was removed, non-obliterated ears were used as control and granular (Polybone-G) or powder (Polybone-P) types of Polybone (tricalicium phosphate and polyphosphate) were applied into the dorsal bulla. The radiological and histologic findings were obtained from each animal at 8 and 20 weeks after obliteration.
On radiological examination, thickening of the bulla wall observed in all experimental groups compared with normal group. Increased homogenous bony densities continuous with the bulla wall were observed in the Polybone-G group compared to the other experimental groups, and some isolated bony densities were observed in the Polybone-P group. Histopathologic findings showed increased new bone formations and less inflammatory reactions in the Polybone-G group compared to the Polybone-P group.
This study demonstrates that granular type calcium phosphate can be used as effective material than powder type in mastoid obliteration.
本研究旨在以豚鼠为动物模型，评估乳突根治术后不同类型磷酸钙的适用性。
26只雄性豚鼠用于实验研究。4只豚鼠作为正常对照组，其余用于实验组。去除鼓泡黏膜后，将未行根治术的耳朵作为对照，把颗粒型（Polybone - G）或粉末型（Polybone - P）的Polybone（磷酸三钙和多聚磷酸盐）应用于背侧鼓泡。在根治术后8周和20周从每只动物获取放射学和组织学结果。
放射学检查显示，与正常组相比，所有实验组的鼓泡壁均增厚。与其他实验组相比，Polybone - G组观察到与鼓泡壁连续的均匀骨密度增加，而Polybone - P组观察到一些孤立的骨密度。组织病理学结果显示，与Polybone - P组相比，Polybone - G组新骨形成增加且炎症反应减少。
本研究表明，在乳突根治术中，颗粒型磷酸钙比粉末型可作为更有效的材料。
