Ghanei Mostafa, Chilosi Marco, Mohammad Hosseini Akbari Hassan, Motiei-Langroudi Rouzbeh, Harandi Ali Amini, Shamsaei Hassan, Bahadori Moslem, Tazelaar Henry D
Research Center of Chemical Injuries, Baqiyatallah Medical Science University, Mollasadra Street, P.O. Box: 19945-546 , Tehran, Iran.
Patholog Res Int. 2011;2011:659603. doi: 10.4061/2011/659603. Epub 2011 Jul 6.
We performed a pathologic study with further using an immunohistochemical technique (using anti-p63 and anti-CK5) on tissues obtained by open lung biopsy from 18 patients with previous exposure to sulphur mustard (SM) as case group and 8 unexposed patients (control group). The most frequent pathologic diagnosis was constrictive bronchiolitis (44.4%), followed by respiratory (22.2%) and chronic cellular bronchiolitis (16.7%) in the case group, and hypersensitivity bronchiolitis (50%) in the control group. The pathologic diagnoses were significantly different in the case and control groups (P = 0.042). In slides stained by anti-p63 and anti-CK5, the percent of stained cells and the mean number of epithelial cells were lower in the case group in comparison to the control group. This difference was significant for the mean number of cells stained by anti-CK5 (P = 0.042). Furthermore, there was a significant correlation between pathologic diagnosis and total number of cells and mean number of cells stained with anti-p63 and anti-CK5 (P value = 0.002, <0.001, 0.044). These results suggest that constrictive bronchiolitis may be the major pathologic consequence of exposure to SM. Moreover, decrease of p63 in respiratory tissues affected by SM may suggest the lack of regenerative capacity in these patients.
我们进行了一项病理学研究，进一步采用免疫组织化学技术（使用抗p63和抗CK5），对18例曾接触硫芥（SM）的患者（病例组）经开放性肺活检获取的组织以及8例未接触者（对照组）的组织进行检测。病例组最常见的病理诊断为缩窄性细支气管炎（44.4%），其次为呼吸性细支气管炎（22.2%）和慢性细胞性细支气管炎（16.7%），对照组为过敏性细支气管炎（50%）。病例组和对照组的病理诊断存在显著差异（P = 0.042）。在抗p63和抗CK5染色的切片中，病例组染色细胞百分比和上皮细胞平均数低于对照组。抗CK5染色细胞平均数的差异具有统计学意义（P = 0.042）。此外，病理诊断与抗p63和抗CK5染色的细胞总数及平均数之间存在显著相关性（P值分别为0.002、<0.001、0.044）。这些结果表明，缩窄性细支气管炎可能是接触SM的主要病理后果。此外，受SM影响的呼吸组织中p63减少可能提示这些患者缺乏再生能力。
