Crawford Matthew J, Rapireddy Srinivas, Bahal Raman, Sacui Iulia, Ly Danith H
Department of Chemistry, Center for Nucleic Acids Science and Technology (CNAST), Mellon Institute, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
J Nucleic Acids. 2011;2011:652702. doi: 10.4061/2011/652702. Epub 2011 May 25.
Conformationally preorganized peptide nucleic acids (PNAs) have been synthesized through backbone modifications at the γ-position, where R = alanine, valine, isoleucine, and phenylalanine side chains. The effects of these side-chains on the conformations and hybridization properties of PNAs were determined using a combination of CD and UV-Vis spectroscopic techniques. Our results show that the γ-position can accommodate varying degrees of sterically hindered side-chains, reaffirming the bimodal function of PNAs as the true hybrids of "peptides" and "nucleic acids."
通过在γ位进行主链修饰合成了构象预组织的肽核酸（PNA），其中R为丙氨酸、缬氨酸、异亮氨酸和苯丙氨酸侧链。使用圆二色光谱（CD）和紫外可见光谱（UV-Vis）技术相结合的方法，确定了这些侧链对PNA构象和杂交特性的影响。我们的结果表明，γ位可以容纳不同程度的空间位阻侧链，再次证实了PNA作为“肽”和“核酸”真正杂合体的双峰功能。
