人类淋巴细胞和白血病细胞上的生长抑素受体。
Somatostatin receptors on human lymphocytes and leukaemia cells.
作者信息
Hiruma K, Koike T, Nakamura H, Sumida T, Maeda T, Tomioka H, Yoshida S, Fujita T
机构信息
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
出版信息
Immunology. 1990 Dec;71(4):480-5.
Receptors for somatostatin were identified on mitogen-activated human peripheral blood lymphocytes (PBL) and human leukaemic cells in 87.5% of lymphoblastic leukaemia and in 12.5% of non-lymphocytic leukaemia, using a somatostatin radiobinding assay. The specific binding of 125I-somatostatin of these cells increased linearly with the cell numbers and was suppressed by non-iodinated somatostatin. We investigated the distribution of fluorescent somatostatin to mitogen-activated PBL by using a fluorescence-activated cell sorter (FACS). Over 95% of the cell populations bound fluorescent somatostatin and no distinct predilection was found among certain lymphocyte subpopulations and somatostatin receptor-positive cells. Scatchard analysis showed a single class (low affinity) of binding site on mitogen-activated PBL and two classes (high and low affinity) of specific binding sites on lymphoblastic leukaemia cells.
采用生长抑素放射结合试验，在促有丝分裂激活的人外周血淋巴细胞（PBL）和人白血病细胞上鉴定出了生长抑素受体，在87.5%的淋巴细胞白血病和12.5%的非淋巴细胞白血病中均可检测到。这些细胞对125I-生长抑素的特异性结合随细胞数量呈线性增加，并被非碘化生长抑素所抑制。我们使用荧光激活细胞分选仪（FACS）研究了荧光生长抑素在促有丝分裂激活的PBL中的分布。超过95%的细胞群体结合了荧光生长抑素，在某些淋巴细胞亚群和生长抑素受体阳性细胞之间未发现明显的偏好性。Scatchard分析显示，促有丝分裂激活的PBL上有一类（低亲和力）结合位点，淋巴细胞白血病细胞上有两类（高亲和力和低亲和力）特异性结合位点。
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