Thierry D, Brisson-Noël A, Vincent-Lévy-Frébault V, Nguyen S, Guesdon J L, Gicquel B
Laboratoire des Sondes Froides, Centre National de la Recherche Scientifique Unité de Recherche Associée No. 1300, Institut Pasteur, Paris, France.
J Clin Microbiol. 1990 Dec;28(12):2668-73. doi: 10.1128/jcm.28.12.2668-2673.1990.
An insertion sequence-like element, IS6110, was isolated from a Mycobacterium tuberculosis cosmid library as a repetitive sequence. IS6110 shows similarities with elements of the IS3 family. This insertion sequence was found to be specific to mycobacteria belonging to the M. tuberculosis complex. For detection and identification of M. tuberculosis bacilli in uncultured specimens, oligonucleotides derived from the IS6110 sequence were used as primers and probes in polymerase chain reaction studies. The results obtained were consistent with results of classical identification procedures, bacteriological data, and clinical criteria.
一种类插入序列元件IS6110，作为重复序列从结核分枝杆菌黏粒文库中分离出来。IS6110与IS3家族的元件具有相似性。发现该插入序列是结核分枝杆菌复合群分枝杆菌所特有的。为了在未培养的标本中检测和鉴定结核分枝杆菌，源自IS6110序列的寡核苷酸被用作聚合酶链反应研究中的引物和探针。获得的结果与经典鉴定程序、细菌学数据和临床标准的结果一致。
