Charlton J A, Baylis P H
Department of Medicine, Medical School, University of Newcastle upon Tyne.
J Endocrinol. 1990 Dec;127(3):377-82. doi: 10.1677/joe.0.1270377.
Previous studies have indicated that ornithine decarboxylase (ODC) may be involved in the stimulation of Na+/K(+)-ATPase activity by arginine vasopressin (AVP) in the rat renal medullary thick ascending limb of Henle's loop. The present study was aimed at establishing the role of the polyamines, the conversion products of ODC activity, in the stimulation of Na+/K(+)-ATPase by AVP. Using cytochemical methods, we have demonstrated an increase in Na+/K(+)-ATPase activity after stimulation with putrescine, spermidine and spermine (each 1 mmol/l) for 2.5, 2 and 1.5 min respectively. The specific inhibitors of spermidine and spermine synthase, bis-cyclohexylammonium sulphate and N-alkylated-1,3-diaminopropane respectively, inhibited the stimulation of Na+/K(+)-ATPase by AVP, this inhibition being reversed by spermine. These findings suggest that polyamines are involved in the stimulus-response coupling of the hormone-mediated response.
先前的研究表明，鸟氨酸脱羧酶（ODC）可能参与精氨酸血管加压素（AVP）对大鼠肾髓质亨氏袢厚升支中Na+/K(+)-ATP酶活性的刺激作用。本研究旨在确定ODC活性的转化产物多胺在AVP刺激Na+/K(+)-ATP酶过程中的作用。使用细胞化学方法，我们证明分别用腐胺、亚精胺和精胺（各1 mmol/l）刺激2.5、2和1.5分钟后，Na+/K(+)-ATP酶活性增加。亚精胺和精胺合酶的特异性抑制剂，分别为硫酸双环己基铵和N-烷基化-1,3-二氨基丙烷，抑制了AVP对Na+/K(+)-ATP酶的刺激作用，精胺可逆转这种抑制作用。这些发现表明多胺参与了激素介导反应的刺激-反应偶联。
