Charlton J A, Baylis P H
Department of Medicine, Medical School, Newcastle-upon-Tyne, U.K.
Cell Biochem Funct. 1990 Jan;8(1):25-9. doi: 10.1002/cbf.290080105.
The anti-diuretic hormone, arginine vasopressin (AVP) stimulates the activity of Na+K+ATPase in the rat renal medullary thick ascending limb of Henle's loop (mTAL). Atrial natriuretic factor (ANF) has been suggested to exert a tubular effect on the mammalian nephron, perhaps in part, by interacting with other hormones. In the present study, we investigated the effect of rat ANF with and without AVP upon mTAL Na+K+ATPase activity using cytochemical methods. ANF alone failed to inhibit or stimulate Na+K+ATPase activity in mTAL at any of the concentrations tested (10 nmol-0.1 pmol l-1). Unlike the rat hypothalamic digitalis-like factor, ANF (10 nmol-10 fmol l-1) did not inhibit Na+K+ATPase activity after stimulation with AVP (1 fmol l-1) for either 4 or 10 min. The results suggest that ANF does not exert an effect on mTAL, either alone or in conjunction with AVP.
抗利尿激素，即精氨酸加压素（AVP），可刺激大鼠肾髓质亨氏袢升支粗段（mTAL）中Na+K+ATP酶的活性。有人提出，心房利钠因子（ANF）可能部分通过与其他激素相互作用，对哺乳动物肾单位产生肾小管效应。在本研究中，我们使用细胞化学方法研究了有无AVP时大鼠ANF对mTAL中Na+K+ATP酶活性的影响。在所测试的任何浓度（10 nmol - 0.1 pmol l-1）下，单独的ANF均未能抑制或刺激mTAL中的Na+K+ATP酶活性。与大鼠下丘脑类洋地黄因子不同，ANF（10 nmol - 10 fmol l-1）在AVP（1 fmol l-1）刺激4分钟或10分钟后，并未抑制Na+K+ATP酶活性。结果表明，ANF单独或与AVP联合使用时，均不会对mTAL产生影响。
