Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel.
Bioorg Med Chem. 2012 Jan 15;20(2):554-70. doi: 10.1016/j.bmc.2011.06.066. Epub 2011 Jun 29.
Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.
重氮甲烷是最小的光反应基团之一，在光照下会形成反应性卡宾。这一特性已被广泛应用于光亲和标记，以研究配体-受体、配体-酶和蛋白质-蛋白质相互作用，并用于分离和鉴定未知蛋白质。本文综述了重氮甲烷在光亲和标记中的最新进展。
