Office of Research, Evaluation and Surveillance, Controlled Substance and Tobacco Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada.
Nicotine Tob Res. 2011 Nov;13(11):1045-51. doi: 10.1093/ntr/ntr127. Epub 2011 Jul 20.
Validation of smoking status is a vital component for assessing self-reported smoking status. This study examined commercially available urinary cotinine immunoassay strips to evaluate their effectiveness and their reliability in validating self-reported tobacco usage.
This study compared cotinine values from urinary test strips and liquid chromatography-mass spectrometry (LC-MS/MS) obtained from 100 self-reported nonsmokers and 201 self-reported smokers.
The analyzed test strips, with 100 ng/ml as the cutoff value, have 91% (84%-96% CI) specificity and 92% (86%-95% CI) sensitivity. The test strips successfully identified nonsmokers from smokers with excellent reproducibility. However, self-reported smokers have significantly different (p < .001) test strip results when compared with the expected LC-MS/MS values.
Overall, the test strip demonstrated that it discriminates nonsmokers from smokers and is an adept alternative to chromatographic methods.
验证吸烟状况是评估自我报告吸烟状况的一个重要组成部分。本研究使用市售的尿液可替宁免疫检测条评估其有效性和可靠性，以验证自我报告的烟草使用情况。
本研究比较了 100 名自我报告的非吸烟者和 201 名自我报告的吸烟者的尿液检测条和液相色谱-质谱（LC-MS/MS）的可替宁值。
分析的检测条以 100ng/ml 为截断值，具有 91%（86%-95%CI）的特异性和 92%（84%-96%CI）的敏感性。这些检测条具有出色的重现性，可成功地将非吸烟者与吸烟者区分开来。然而，与预期的 LC-MS/MS 值相比，自我报告的吸烟者的检测条结果有显著差异（p<.001）。
总的来说，该检测条能够区分非吸烟者和吸烟者，是一种替代色谱方法的有效手段。
