岐阜市冬季与急性胃肠炎相关的A组人轮状病毒血清型分析

Serotype analysis of group A human rotavirus related to acute gastroenteritis in winter in Gifu city.

作者信息

Kawamoto H, Tanaka H, Urasawa S, Urasawa T, Taniguchi K

机构信息

Department of Virology, Gifu Prefectural Institute of Public Health.

出版信息

Microbiol Immunol. 1990;34(8):675-81. doi: 10.1111/j.1348-0421.1990.tb01043.x.

DOI:10.1111/j.1348-0421.1990.tb01043.x

PMID:2177831

Abstract

A total of 348 fecal specimens collected from children with acute gastroenteritis in Gifu city over three consecutive winter seasons (from November 1986 to March 1989) were examined for group A human rotavirus (HRV) by either a commercial test kit or a sandwich enzyme-linked immunosorbent assay (sandwich ELISA). One hundred twelve of the 173 group A HRV-positive specimens were further subjected to serotype determination by ELISA with four serotype-specific monoclonal antibodies to VP7 (ELISA-serotyping). Ninety-one specimens (81.3%) were successfully serotyped: 41 (36.6%) were serotype 1, 13 (11.6%) serotype 2, 27 (24.1%) serotype 3, and 10 (8.9%) serotype 4. The serotypes of the remaining 21 (18.8%) could not be determined. The predominant serotype of HRV that prevailed in Gifu city changed every winter: serotype 3 (63.4%) was most prevalent in the 1st winter, serotype 4 (42.9%) in the 2nd winter, and serotype 1 (64%) in the 3rd winter.

摘要

在连续三个冬季（从1986年11月至1989年3月）期间，从岐阜市患有急性肠胃炎的儿童中总共收集了348份粪便样本，使用商用检测试剂盒或夹心酶联免疫吸附测定法（夹心ELISA）对A组人轮状病毒（HRV）进行检测。在173份A组HRV阳性样本中，有112份进一步使用针对VP7的四种血清型特异性单克隆抗体通过ELISA进行血清型测定（ELISA血清分型）。91份样本（81.3%）成功进行了血清分型：41份（36.6%）为1型，13份（11.6%）为2型，27份（24.1%）为3型，10份（8.9%）为4型。其余21份样本（18.8%）的血清型无法确定。岐阜市流行的HRV优势血清型每年冬季都有所变化：在第一个冬季，3型（63.4%）最为流行；在第二个冬季，4型（42.9%）最为流行；在第三个冬季，1型（64%）最为流行。