马来西亚不同地区轮状病毒的血清型分析。
Serotype analysis of rotaviruses from different locations in Malaysia.
作者信息
Rasool N B, Green K Y, Kapikian A Z
机构信息
Department of Genetics and Cellular Biology, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
J Clin Microbiol. 1993 Jul;31(7):1815-9. doi: 10.1128/jcm.31.7.1815-1819.1993.
The distribution of rotavirus G (VP7) serotypes circulating in four locations in Malaysia, representing three geographical areas, was evaluated in 341 RNA-positive stool specimens obtained discontinuously between 1977 and 1988 from infants and young children under the age of five years who were hospitalized with acute gastroenteritis. A total of 306 specimens (256 stool suspensions and 50 that were adapted to growth in tissue culture) that were rotavirus positive by the confirmatory enzyme-linked immunosorbent assay (ELISA) were examined for serotype by ELISA utilizing monoclonal antibodies to rotavirus G serotype 1, 2, 3, 4, or 9. One hundred eighty (59%) of the 306 specimens could be serotyped; of these 180 specimens, 71% were serotype 4, 15% were serotype 1, 4% were serotype 2, and 4% were serotype 3. Serotype 9 rotavirus was not detected. Most (71%) of the specimens tested were obtained in 1988, when serotype 4 predominated in three locations in West Malaysia; no single serotype was predominant in a limited number of specimens from East Malaysia.
对1977年至1988年间从因急性胃肠炎住院的5岁以下婴幼儿中间断采集的341份RNA阳性粪便标本进行评估，以确定代表三个地理区域的马来西亚四个地点流行的轮状病毒G（VP7）血清型分布情况。通过使用针对轮状病毒G血清型1、2、3、4或9的单克隆抗体的酶联免疫吸附测定（ELISA），对总共306份经确证ELISA检测为轮状病毒阳性的标本（256份粪便悬液和50份适应于在组织培养中生长的标本）进行血清型检测。306份标本中有180份（59%）可以进行血清分型；在这180份标本中，71%为血清型4，15%为血清型1，4%为血清型2，4%为血清型3。未检测到血清型9轮状病毒。大多数（71%）检测标本是在1988年获得的，当时血清型4在马来西亚西部的三个地点占主导地位；在来自东马来西亚的数量有限的标本中，没有单一血清型占主导地位。
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