Böhm Gabriele, Ushakova Yelena, Alizai Hamid Patrick, Braunschweig Till, Lente Christina, Heffels Karl-Heinz, Groll Jürgen, Neumann Ulf Peter, Junge Karsten
Department of Surgery, University Hospital, Technical University of Aachen, Aachen, Germany.
Eur Surg Res. 2011;47(3):118-29. doi: 10.1159/000329412. Epub 2011 Jul 20.
In order to allow inflammatory response modification and ultimately improvement in tissue remodeling, we developed a new surface modification for meshes that will serve as a carrier for other substances. Biocompatibility is tested in an animal model.
The animal model for diaphragmatic hernia repair was established in prior studies. Meshes were surface modified with star-configured PEO (polyethylene oxide)-based molecules [sP(EO-stat-PO)]. An electrospun nanoweb of short-term absorbable PLGA (polylactide-co-glycolide) with integrated sP(EO-stat-PO) molecules was applied onto the modified meshes. This coating also served as aerial sealing of the diaphragm. A final layer of hydrogel was applied to the product. Adhesive properties, defect size and mesh shrinkage were determined, and histological and immunohistochemical investigations performed after 4 months.
The mean defect size decreased markedly in both modified mesh groups. Histologically and with regard to apoptosis and proliferation rate, smooth muscle cells, collagen I/III ratio and macrophage count, no statistically significant difference was seen between the 3 mesh groups.
In this proof-of-principle investigation, we demonstrate good biocompatibility for this surface-modified mesh compared to a standard polypropylene-based mesh. This new coating represents a promising tool as a carrier for bioactive substances in the near future.
为了实现炎症反应调节并最终改善组织重塑，我们开发了一种用于网片的新表面改性方法，该网片将作为其他物质的载体。在动物模型中测试生物相容性。
在先前的研究中建立了膈疝修复的动物模型。用星形结构的基于聚环氧乙烷（PEO）的分子[sP(EO-stat-PO)]对网片进行表面改性。将具有整合的sP(EO-stat-PO)分子的短期可吸收聚乳酸-乙醇酸共聚物（PLGA）的电纺纳米网应用于改性网片上。该涂层还用作膈肌的气密封闭。在产品上施加最后一层水凝胶。测定粘附性能、缺损大小和网片收缩情况，并在4个月后进行组织学和免疫组织化学研究。
两个改性网片组的平均缺损大小均显著减小。在组织学以及细胞凋亡和增殖率、平滑肌细胞、I/III型胶原蛋白比例和巨噬细胞计数方面，3个网片组之间未见统计学显著差异。
在这项原理验证研究中，与标准聚丙烯基网片相比，我们证明了这种表面改性网片具有良好的生物相容性。这种新涂层在不久的将来作为生物活性物质的载体是一种很有前景的工具。
