Arnold K, Flanagan T D, Ohki S
Karl Marx University, Medical School, Institute of Biophysics, Leipzig, GDR.
Biomed Biochim Acta. 1990;49(7):633-5.
Liposomes made from phosphatidylserine and labeled with the fluorescence probe pyrene-phosphatidylcholine were used for studies of the influence of dextran sulfate on the fusion process. The fusion is monitored by the increase of the fluorescence signal due to the dilution of the fluorescence probes as a result of the fusion of liposomes with the virus. Addition of dextran sulfate inhibits the fusion process. Binding of the sulfate groups of the polymer to aminogroups on the virus proteins is considered as the reason for the inhibiting effect of this polymer on fusion.
由磷脂酰丝氨酸制成并用荧光探针芘 - 磷脂酰胆碱标记的脂质体用于研究硫酸葡聚糖对融合过程的影响。通过由于脂质体与病毒融合导致荧光探针稀释而引起的荧光信号增加来监测融合。添加硫酸葡聚糖会抑制融合过程。该聚合物的硫酸基团与病毒蛋白上的氨基结合被认为是该聚合物对融合产生抑制作用的原因。
