Ohki S, Arnold K, Srinivasakumar N, Flanagan T D
Department of Biophysical Sciences, State University of New York, Buffalo 14214.
Biomed Biochim Acta. 1991;50(2):199-206.
The effect of dextran sulfate on the fusion of Sendai virus and erythrocyte ghosts was studied as a function of dextran sulfate concentration and pH of cell suspension solutions. In order to examine the interaction of dextran sulfate with Sendai virion and erythrocyte ghost surfaces, the turbidity of cell suspensions was also measured with respect to the same parameters as above. It was found that dextran sulfate inhibited the fusion of Sendai virus with erythrocyte ghosts. The lower the pH of the suspension solution was, the more effective was dextran sulfate in suppressing virion erythrocyte ghost fusion. Dextran sulfate of a higher molecular weight was slightly more effective in suppressing fusion than that of a lower molecular weight. From turbidity measurements of Sendai virus and erythrocyte ghost suspensions, it is likely that dextran sulfate interacts preferentially with Sendai virion surfaces and inhibits interaction between Sendai virions and erythrocyte ghosts.
研究了硫酸葡聚糖对仙台病毒与红细胞血影融合的影响，该影响是硫酸葡聚糖浓度和细胞悬液pH值的函数。为了检测硫酸葡聚糖与仙台病毒粒子及红细胞血影表面的相互作用，还针对上述相同参数测量了细胞悬液的浊度。结果发现，硫酸葡聚糖抑制仙台病毒与红细胞血影的融合。悬液的pH值越低，硫酸葡聚糖抑制病毒粒子与红细胞血影融合的效果就越显著。较高分子量的硫酸葡聚糖在抑制融合方面比低分子量的硫酸葡聚糖稍有效。根据对仙台病毒和红细胞血影悬液的浊度测量结果，硫酸葡聚糖可能优先与仙台病毒粒子表面相互作用，并抑制仙台病毒粒子与红细胞血影之间的相互作用。
