Monstein H J
Dept. of Clinical Chemistry, Laboratory of Molecular Biology, Copenhagen, Denmark.
Biosci Rep. 1990 Oct;10(5):461-7. doi: 10.1007/BF01152293.
A 1000 base pair cDNA coding for the entire human proenkephalin A(proA) polypeptide was subcloned into the multifunctional pMPV 2911/M E. coli vector. The recombinant plasmid was found to express an approximately 30 kDa prohormone, which was recognized by a Met-Arg6-Phe2 antibody, directed against the C-terminal part of the enkephalin A prohormone. The expression of human proenkephalin A cDNA should thus permit the rapid purification of unfused recombinant enkephalin A prohormone, which itself may provide a model substrate to identify endoproteolytic processing activities.
