Thomas G, Herbert E, Hruby D E
Science. 1986 Jun 27;232(4758):1641-3. doi: 10.1126/science.3754979.
The posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. The infected cells rapidly produced both cellular and secreted Met-enkephalin immunoreactivity. Although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. The quantity of intact proenkephalin secreted from BSC-40 cells (derived from African Green monkey kidney) was sufficient to establish the value of vaccinia virus as a mammalian cell expression vector.
通过用表达人前脑啡肽的重组痘苗病毒感染多种细胞类型，评估了复杂前体多蛋白人前脑啡肽的翻译后成熟过程。被感染的细胞迅速产生细胞内和分泌型甲硫氨酸脑啡肽免疫反应性。尽管每个细胞系都能分泌完整的前脑啡肽，但只有小鼠垂体细胞系能够将前脑啡肽加工成成熟的脑啡肽肽段。从BSC - 40细胞（源自非洲绿猴肾）分泌的完整前脑啡肽的量足以确定痘苗病毒作为哺乳动物细胞表达载体的价值。
