Department of Life System Science, Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, Shobara, Hiroshima, Japan.
J Basic Microbiol. 2012 Apr;52(2):132-40. doi: 10.1002/jobm.201100014. Epub 2011 Jul 21.
The sdhB gene, encoding the iron-sulfur protein (Ip) subunit of succinate dehydrogenase (Sdh, EC 1.3.99.1), has been cloned from the violet root rot fungus, Helicobasidium mompa, and characterized. The promoter region contains a CCAAT box, TATA-like box, and CT-rich region. The gene is interrupted by eight introns and is predicted to encode a polypeptide of 291 amino acid residues. The putative amino acid sequence of the encoded product of sdhB gene from H. mompa shows high homology to the other known sdhB genes and is 79% identical to the Ip subunit of SdhB of Uromyces fabae. Three cysteine-rich clusters associated with the iron-sulfur centers involved in electron transport were particularly well conserved. One of these clusters contains a critical histidine residue implicated in carboxin sensitivity in the basidiomycetes. Only one copy of the gene was present in the genome of H. mompa, and reverse transcription (RT)-PCR analysis of mRNA expression showed that the sdhB gene was transcribed in potato dextrose broth.
已从紫罗兰根腐病菌 Helicobasidium mompa 中克隆并鉴定了编码琥珀酸脱氢酶（Sdh，EC 1.3.99.1）铁硫蛋白（Ip）亚基的 sdhB 基因。启动子区域包含 CCAAT 盒、TATA 样盒和富含 CT 的区域。该基因被 8 个内含子中断，并预测编码 291 个氨基酸残基的多肽。H. mompa 的 sdhB 基因编码产物的推定氨基酸序列与其他已知的 sdhB 基因高度同源，与 Uromyces fabae 的 SdhB 的 Ip 亚基有 79%的同一性。与电子传递中涉及的铁硫中心相关的三个富含半胱氨酸的簇特别保守。其中一个簇含有一个关键的组氨酸残基，该残基与担子菌中的羧基肟敏感性有关。H. mompa 的基因组中只有一个基因拷贝，对 mRNA 表达的逆转录（RT）-PCR 分析表明 sdhB 基因在马铃薯葡萄糖肉汤中转录。
