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Cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase from Pleurotus ostreatus.

作者信息

Irie T, Honda Y, Matsuyama T, Watanabe T, Kuwahara M

机构信息

Wood Research Institute, Kyoto University, Japan.

出版信息

Biochim Biophys Acta. 1998 Mar 4;1396(1):27-31. doi: 10.1016/s0167-4781(97)00203-0.

DOI:10.1016/s0167-4781(97)00203-0
PMID:9524211
Abstract

Genomic and cDNA fragments encoding the iron-sulfur protein (Ip) subunit of dehydrogenase (EC 1.3.99.1) have been cloned from the edible basidiomycetous fungus, Pleurotus ostreatus. The gene is interrupted by five introns and is predicted to encode a polypeptide of 268 amino acid residues. Sequence comparison with the Ip subunit from other species identified three conserved cysteine-rich clusters. One of these contains a critical histidine residue implicated in carboxin sensitivity in the heterobasidiomycete Ustilago maydis.

摘要

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