Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, South Korea.
Phytother Res. 2012 Feb;26(2):284-90. doi: 10.1002/ptr.3545. Epub 2011 Jul 21.
Duchesnea chrysantha belongs to the Rosaceae family and has been used traditionally for the treatment of various diseases in Korea and other parts of East Asia. This study examined the antiinflammatory effect of Duchesnea chrysantha extract (DcE) on atopic dermatitis in vitro and in vivo. DcE inhibited the production of IL-6, IL-8 and MCP-1 in THP-1 cells and the release of IL-6 and MCP-1 in EoL-1 cells after treatment with house dust mite extract. In the in vivo experiment, Nc/Nga mice were sensitized to DNCB and then orally and dorsally administered DcE (50 mg/kg in PBS) for 3 weeks. The DcE administration significantly reduced the skin severity score when compared with the control group and inhibited the thickening of the epidermis and infiltration of inflammatory cells into the dermis. In addition, the serum IgE levels decreased markedly in the DcE-treated mice when compared with the control group. The synthesis of IL-5, IL-13, MCP-1 and eotaxin was also decreased in splenocytes of the DcE-treated group, while IFN-γ was increased in the Dc-administered group. These results may indicate that DcE attenuates the development of atopic dermatitis-like lesions by lowering the IgE and inflammatory cytokine levels, and that it is useful in drug development for the treatment of atopic dermatitis.
软枣猕猴桃属于蔷薇科，在韩国和东亚其他地区，传统上被用于治疗各种疾病。本研究考察了软枣猕猴桃提取物（DcE）对特应性皮炎的体外和体内抗炎作用。DcE 可抑制 THP-1 细胞中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和单核细胞趋化蛋白-1（MCP-1）的产生，以及 EoL-1 细胞中屋尘螨提取物处理后 IL-6 和 MCP-1 的释放。在体内实验中，Nc/Nga 小鼠对 DNCB 致敏，然后用 DcE（PBS 中的 50mg/kg）经口和背部给药 3 周。与对照组相比，DcE 给药可显著降低皮肤严重程度评分，并抑制表皮增厚和真皮炎性细胞浸润。此外，与对照组相比，DcE 治疗组的血清 IgE 水平显著降低。DcE 治疗组脾细胞中白细胞介素-5（IL-5）、白细胞介素-13（IL-13）、MCP-1 和嗜酸性粒细胞趋化因子（eotaxin）的合成也减少，而 IFN-γ 增加。这些结果可能表明，DcE 通过降低 IgE 和炎症细胞因子水平，减轻特应性皮炎样病变的发展，可用于特应性皮炎治疗药物的开发。
