Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.
J Ethnopharmacol. 2012 Oct 31;144(1):151-9. doi: 10.1016/j.jep.2012.08.042. Epub 2012 Sep 3.
Illicium verum is a traditional herbal medicine with anti-inflammatory properties used in Asia. However, its usefulness in the treatment of allergic diseases remains unclear. This study evaluated the anti-inflammatory and antiallergic effects of I. verum extract (IVE) in a mouse model of atopic dermatitis.
We investigated the effects of IVE on compound 48/80-induced histamine release, and phorbol 12-myristate13-acetate and calcium ionophore A23187-stimulated cytokines secretion in MC/9 mast cells. Atopic dermatitis was induced in NC/Nga mice by exposure to extract of house dust mite (Dermatophagoides farinae). After a topical application of IVE on ear and skin lesions, we evaluated the severity of skin symptoms, ear thickness, inflammatory cell infiltration, and serum levels of immunoglobulin E (IgE), histamine, interleukin (IL)-6, and intercellular adhesion molecule (ICAM)-1. In addition, we determined the expression of IL-4, IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ thymus- and activation-regulated chemokine (TARC), regulated on activation, normal T cell expressed and secreted (RANTES), ICAM-1, and vascular cell adhesion molecule (VCAM)-1 in ear tissues.
IVE inhibited secretion of histamine, IL-4, IL-6, and TNF-α from mast cells in a dose-dependent manner. Topical application of IVE significantly reduced dermatitis scores, ear thickness, and serum levels of IgE, histamine, IL-6, and ICAM-1. Histopathological analysis demonstrated decreased epidermal thickening and dermal infiltration by inflammatory cells. In the ear lesions, IVE treatment reduced expression of IL-4, IL-6, TNF-α, TARC, RANTES, ICAM-1, and VCAM-1, but not IFN-γ.
These results indicate that IVE inhibits atopic dermatitis-like skin lesions by suppressing the expression of cytokines, chemokines, and adhesion molecules. These results suggest that IVE may be a potential therapeutic candidate for atopic dermatitis.
八角属植物是一种具有抗炎特性的传统草药，在亚洲被用于治疗多种疾病。然而，其在治疗过敏性疾病方面的有效性尚不清楚。本研究评估了八角属植物提取物（IVE）在特应性皮炎小鼠模型中的抗炎和抗过敏作用。
我们研究了 IVE 对化合物 48/80 诱导的组胺释放以及佛波醇 12-肉豆蔻酸 13-乙酸酯和钙离子载体 A23187 刺激的 MC/9 肥大细胞细胞因子分泌的影响。通过暴露于屋尘螨（屋尘螨）提取物在 NC/Nga 小鼠中诱导特应性皮炎。在耳和皮肤损伤部位局部应用 IVE 后，我们评估了皮肤症状严重程度、耳厚度、炎症细胞浸润以及血清免疫球蛋白 E（IgE）、组胺、白细胞介素（IL）-6 和细胞间黏附分子（ICAM）-1 水平。此外，我们还测定了耳组织中 IL-4、IL-6、肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ胸腺激活调节趋化因子（TARC）、调节激活正常 T 细胞表达和分泌（RANTES）、ICAM-1 和血管细胞黏附分子（VCAM）-1 的表达。
IVE 呈剂量依赖性抑制肥大细胞中组胺、IL-4、IL-6 和 TNF-α的分泌。局部应用 IVE 可显著降低特应性皮炎评分、耳厚度和血清 IgE、组胺、IL-6 和 ICAM-1 水平。组织病理学分析显示表皮增厚和真皮炎症细胞浸润减少。在耳损伤部位，IVE 治疗降低了 IL-4、IL-6、TNF-α、TARC、RANTES、ICAM-1 和 VCAM-1 的表达，但 IFN-γ的表达未降低。
这些结果表明，IVE 通过抑制细胞因子、趋化因子和黏附分子的表达抑制特应性皮炎样皮肤损伤。这些结果表明，IVE 可能是特应性皮炎的一种潜在治疗候选药物。
