Shanmugam Kumaran, Yoshinobu Tatsuo, Moon Wonchul, Iwasaki Hiroshi
Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur 613403, Tamil Nadu, India.
J Nanosci Nanotechnol. 2011 May;11(5):3808-13. doi: 10.1166/jnn.2011.4171.
Oxide dots fabricated on silicon (111) by the Atomic Force Microscopy (AFM) anodic oxidation technique was used for the patterning of two different proteins namely, ferritin and fibronectin. Si surfaces were oxidized by the SC1 process and then modified with octadecyltrichlorosilane (OTS) for passivation. Oxide dots were fabricated by applying a bias voltage between the AFM probe and the silicon surface. Furthermore, surface functionalization of oxide dots was achieved through gamma-aminopropyltriethoxysilane (gamma-APTES) and glutaraldehye modification to establish a covalent bond between aldehydes and amino groups of protein molecules. Topographies after each modification steps were monitored by AFM. We were able to achieve positive patterning of ferritin molecules up to an average density of 6 x 10(9)/cm2 on gamma-APTES-covered dots, while 9 x 10(8)/cm2 of ferritin molecules remained on the OTS surface. In contrast to this observation, fibronectin molecules were patterned successfully only on oxide dots, and we did not observe any fibronectin molecules on the OTS surface.
通过原子力显微镜（AFM）阳极氧化技术在硅（111）上制备的氧化点用于两种不同蛋白质（即铁蛋白和纤连蛋白）的图案化。硅表面通过SC1工艺进行氧化，然后用十八烷基三氯硅烷（OTS）进行改性以实现钝化。通过在AFM探针和硅表面之间施加偏置电压来制备氧化点。此外，通过γ-氨丙基三乙氧基硅烷（γ-APTES）和戊二醛改性实现氧化点的表面功能化，以在蛋白质分子的醛基和氨基之间建立共价键。通过AFM监测每个改性步骤后的形貌。我们能够在γ-APTES覆盖的点上实现铁蛋白分子的正图案化，平均密度高达6×10⁹/cm²，而在OTS表面上仍保留9×10⁸/cm²的铁蛋白分子。与该观察结果相反，纤连蛋白分子仅在氧化点上成功图案化，并且我们在OTS表面上未观察到任何纤连蛋白分子。
