Nguyen Phuong-Diem, Son Sang Jun, Min Junhong
J Nanosci Nanotechnol. 2014 Aug;14(8):5646-9. doi: 10.1166/jnn.2014.8784.
This study described the prospect of silica nanotube surface modification in simultaneous detection of pathogenic bacteria by coupling cation exchange magnetic separation with quantum dot labeling. The outer surface of magnetic nanoparticles embedded long silica nanotube (capturing SNT) was modified with poly-L-lysine to serve as a cation exchange magnetic nano-complex to capture and isolate Escherichia coli (E. coli) O157:H7 and Salmonella enteritis typhimurium (S. typhimurium) in aqueous phase. Antibody conjugated quantum dot-embedded short SNT (reporting SNT) was used to simultaneously detect both bacteria, giving a high intensity and photo-stable fluorescent image that can detect single leveled bacterium binding on the capturing SNT. The fluorescent intensity generated from the capturing of both bacteria at different concentration was in the range of 10(2)-10(5) CFU/ml for both E. coli and S. typhimurium.
本研究描述了通过阳离子交换磁分离与量子点标记相结合的方法，对二氧化硅纳米管进行表面改性，用于同时检测病原菌的前景。嵌入长二氧化硅纳米管的磁性纳米颗粒（捕获型SNT）的外表面用聚-L-赖氨酸进行改性，作为阳离子交换磁性纳米复合物，用于在水相中捕获和分离大肠杆菌O157:H7和鼠伤寒沙门氏菌。偶联抗体的嵌入量子点的短SNT（报告型SNT）用于同时检测这两种细菌，可产生高强度且光稳定的荧光图像，能够检测到单个细菌与捕获型SNT的结合。对于大肠杆菌和鼠伤寒沙门氏菌，在不同浓度下捕获这两种细菌所产生的荧光强度范围均为10(2)-10(5) CFU/ml。
