Program in Nutrition and Dietetics and The Medical Food Research and Development Center, Department of Transfusion Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Eur Rev Med Pharmacol Sci. 2011 Jul;15(7):803-8.
OBJECTIVES AND MATERIALS AND METHODS: Medicinal plants constitute an important source of potential therapeutic agents for diabetes and hyperlipidemia. The purpose of present study was to investigate the effect of leaf extract of Moringa oleifera on inhibition of alpha-glucosidase and pancreatic alpha-amylase related to diabetes mellitus. Moreover, the study was also determined in vitro bile acid binding capacity as well as inhibition of cholesterol micellization, pancreatic lipase, and cholesterol esterase activity.
The phytochemical analysis indicated that total phenolic, flavonoid, and condensed tannin contents in the extract were 45.21 +/- 0.96 mg gallic acid equivalents/g extract, 15.39 +/- 0.58 mg catechin equivalents/g extract, and 4.90 +/- 0.20 catechin equivalents/g extract, respectively. In addition, the extract contained a specific inhibitor of intestinal sucrase than intestinal maltase with IC50 value of 0.78 +/- 0.21 mg/ml, whereas it slightly inhibited pancreatic alpha-amylase and pancreatic cholesterol esterase. However, the extract had no inhibitory activity against pancreatic lipase. Furthermore, taurodeoxycholic acid, taurodeoxycholic acid, and glycodeoxycholic acid were bound to the extract (1 mg/ml) with a degree of 26.90 +/- 0.37%, 21.78 +/- 0.68%, and 22.59 +/- 1.02%, respectively. Finally, the extract (10 mg/ml) markedly inhibited the formation of cholesterol micelle about 40.22 +/- 2.64%.
Our results indicated that the leaf extract of Moringa oleifera may be used for the control of blood glucose and lipid concentration and prevention of hyperglycemia and hyperlipidemia.
药用植物是治疗糖尿病和高血脂的潜在治疗剂的重要来源。本研究的目的是研究辣木（Moringa oleifera）叶提取物对抑制与糖尿病相关的α-葡萄糖苷酶和胰腺α-淀粉酶的作用。此外，还测定了体外胆汁酸结合能力以及抑制胆固醇胶束化、胰腺脂肪酶和胆固醇酯酶活性。
植物化学分析表明，提取物中的总酚、类黄酮和缩合单宁含量分别为 45.21 ± 0.96 mg 没食子酸当量/g 提取物、15.39 ± 0.58 mg 儿茶素当量/g 提取物和 4.90 ± 0.20 儿茶素当量/g 提取物。此外，提取物对肠蔗糖酶的抑制作用强于肠麦芽糖酶，IC50 值为 0.78 ± 0.21 mg/ml，而对胰腺α-淀粉酶和胰腺胆固醇酯酶的抑制作用较弱。然而，该提取物对胰腺脂肪酶没有抑制活性。此外，牛磺脱氧胆酸、牛磺胆酸和甘氨脱氧胆酸与提取物（1mg/ml）的结合程度分别为 26.90 ± 0.37%、21.78 ± 0.68%和 22.59 ± 1.02%。最后，提取物（10mg/ml）显著抑制胆固醇胶束的形成，抑制率约为 40.22 ± 2.64%。
我们的研究结果表明，辣木叶提取物可用于控制血糖和血脂浓度，预防高血糖和高血脂。
