Fabiano Adele, Gazzolo Diego, Zimmermann Luc J I, Gavilanes Antonio W D, Paolillo Piermichele, Fanos Vassilios, Caboni Pierluigi, Barberini Luigi, Noto Antonio, Atzori Luigi
Department of Maternal, Fetal and Neonatal Medicine, CasilinoHospital, Rome, Italy.
J Matern Fetal Neonatal Med. 2011 Oct;24 Suppl 2:55-8. doi: 10.3109/14767058.2011.606977.
Metabolomics is a technique used to non-invasively determine a snapshot of the current metabolic status of an organism by analyzing intact tissue or bio-fluids. The aim of the present preliminary study was to analyze metabolic profiles in preterm infants complicated by respiratory distress syndrome (RDS) trough bronchoalveolar lavage fluid (BALF) measurement.
Twelve BALF samples collected at birth prior surfactant, post-surfactant during mechanical ventilation and at extubation time-points, were analyzed by proton nuclear magnetic resonance spectroscopy and Gas chromatography-Mass Spectrometry (GC-MS).
GC-MS analysis identified 25 metabolites of whom 10 had a known molecular structure. They were: undecane, decanoic acid, dodecanoic acid, hexadecanoic acid, octadecanoic acid, hexadecanoic acid methyl ester, 9-octadecanoic acid, tetracosanoic acid, myristic acid, phosphate. These metabolites were over-expressed in BALF collected during mechanical ventilation after surfactant administration.
The present preliminary data suggest that metabolic profile in BALF of RDS infants is becoming possible opening a new cue of metabolomics as promising tool in management of sick premature infants.
代谢组学是一种通过分析完整组织或生物流体来无创确定生物体当前代谢状态快照的技术。本初步研究的目的是通过支气管肺泡灌洗（BALF）测量分析并发呼吸窘迫综合征（RDS）的早产儿的代谢谱。
收集12份在出生时、使用表面活性剂后机械通气期间以及拔管时间点的BALF样本，采用质子核磁共振波谱和气相色谱 - 质谱联用（GC - MS）进行分析。
GC - MS分析鉴定出25种代谢物，其中10种具有已知分子结构。它们是：十一烷、癸酸、十二烷酸、十六烷酸、十八烷酸、十六烷酸甲酯、9 - 十八烷酸、二十四烷酸、肉豆蔻酸、磷酸盐。这些代谢物在使用表面活性剂后机械通气期间收集的BALF中过度表达。
目前的初步数据表明，分析RDS婴儿BALF中的代谢谱成为可能，为代谢组学作为管理患病早产儿的有前景工具开辟了新线索。
