采用代谢组学技术鉴定支气管肺发育不良的新型生物标志物。
Identification of new biomarkers of bronchopulmonary dysplasia using metabolomics.
机构信息
Division of Perinatal Medicine, and Yale Child Health Research Center, Department of Pediatrics, Yale University School of Medicine, New Haven, CT, USA.
Division of Neonatology, Bambino Gesù Children's Hospital, Rome, Italy.
出版信息
Metabolomics. 2019 Feb 2;15(2):20. doi: 10.1007/s11306-019-1482-9.
OBJECTIVE
To identify new biomarkers of bronchopulmonary dysplasia (BPD) in preterm neonates.
STUDY DESIGN
Metabolomic study of prospectively collected tracheal aspirate (TA) samples from preterm neonates admitted in 2 neonatal intensive care units measured by a mass spectroscopy-based assay and analysed using partial least squares-discriminant analysis.
RESULTS
We evaluated 160 TA samples from 68 neonates, 44 with BPD and 24 without BPD in the first week of life. A cluster of 53 metabolites was identified as characteristic of BPD, with 18 select metabolites being highly significant in the separation of BPD versus No BPD. To control for the gestational age (GA) differences, we did a sub-group analyses, and noted that the amino acids histidine, glutamic acid, citrulline, glycine and isoleucine levels were higher in neonates with BPD. In addition, acylcarnitines C16-OH and C18:1-OH were also higher in neonates who developed BPD, but especially in the most preterm infants (neonates with GA < 27 weeks).
CONCLUSION
Metabolomics is a promising approach to identify novel specific biomarkers for BPD.
目的
鉴定早产儿支气管肺发育不良（BPD）的新生物标志物。
研究设计
前瞻性收集入住 2 家新生儿重症监护病房的早产儿气管抽吸（TA）样本，采用基于质谱的检测方法进行代谢组学研究，并使用偏最小二乘判别分析进行分析。
结果
我们评估了来自 68 名新生儿的 160 个 TA 样本，其中 44 名新生儿在生命的第一周患有 BPD，24 名新生儿没有 BPD。鉴定出一组 53 种特征代谢产物与 BPD 有关，其中 18 种选择代谢产物在 BPD 与无 BPD 的分离中具有高度显著性。为了控制胎龄（GA）差异，我们进行了亚组分析，并注意到 BPD 新生儿的组氨酸、谷氨酸、瓜氨酸、甘氨酸和异亮氨酸水平较高。此外，酰基辅酶 A C16-OH 和 C18：1-OH 在发生 BPD 的新生儿中也较高，但在最早产儿（GA<27 周的新生儿）中尤其如此。
结论
代谢组学是一种很有前途的方法，可以鉴定出用于 BPD 的新的特异性生物标志物。
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