Gao Jingqing, Ma Lina, Liu Dianjun, Wang Zhenxin
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Comb Chem High Throughput Screen. 2012 Jan;15(1):90-9. doi: 10.2174/138620712798280808.
In the post-genomic era, glycomics (the functional study of carbohydrates in living organisms) has come into the forefront of biological research because the interactions of glycoconjugates with proteins not only occur widely in biological processes of cells but also initiate infection of host cells by bacteria and viruses. Microarrays have been reportedly successful in carbohydrate-protein interaction as well as cellular surface glycan profiling. This review provides an overview of recent progress in the development of microarray-based techniques for glycomic studies. The fabrication, application and challenge/bottleneck of glycan/lectin microarrays have been summarized and discussed.
在后基因组时代，糖组学（对生物体中碳水化合物的功能研究）已成为生物学研究的前沿领域，因为糖缀合物与蛋白质的相互作用不仅广泛存在于细胞的生物过程中，还引发细菌和病毒对宿主细胞的感染。据报道，微阵列在碳水化合物 - 蛋白质相互作用以及细胞表面聚糖分析方面都取得了成功。本文综述了基于微阵列技术在糖组学研究方面的最新进展。总结并讨论了聚糖/凝集素微阵列的制备、应用以及挑战/瓶颈。
