用于裂殖酵母 bleMX6 抗性选择的 Zeocin。

Zeocin for selection of bleMX6 resistance in fission yeast.

机构信息

Department of Pathology, University of Maryland Medical School, Baltimore, MD 21201, USA.

出版信息

Biotechniques. 2011 Jul;51(1):57-60. doi: 10.2144/000113706.

Abstract

Complementation of auxotrophic nutrient deficiencies in minimal media is widely used for selection of exogenous gene introduction to fission yeast. However, only a limited number of such selection markers are available. Antibiotic resistance markers are good alternatives, but they typically work well in complete rich medium but not in minimal defined Edinburgh minimal medium (EMM). It would be ideal if both the auxotrophic and antibiotic resistance markers can be used together for molecular genetic analysis. Here we describe the use of Zeocin in Pombe minimal glutamate (PMG) media for selection and maintenance of bleMX6 resistance with a LEU2 auxotrophic marker in fission yeast.

摘要

在最小培养基中补充营养缺陷型是广泛用于裂殖酵母中外源基因导入选择的方法。然而，这种选择标记的数量是有限的。抗生素抗性标记是很好的替代品，但它们通常在完全丰富的培养基中效果很好，但在最小定义的爱丁堡最小培养基（EMM）中效果不佳。如果能够同时使用营养缺陷型和抗生素抗性标记来进行分子遗传学分析，那将是非常理想的。在这里，我们描述了在裂殖酵母中使用博来霉素（Zeocin）在 Pombe 最小谷氨酸（PMG）培养基中进行选择和维持 bleMX6 抗性，同时带有 LEU2 营养缺陷型标记。