Prasertsung I, Kanokpanont S, Mongkolnavin R, Wong C S, Panpranot J, Damrongsakkul S
a Department of Chemical Engineering , Faculty of Engineering, Chulalongkorn University , Bangkok , 10330 , Thailand.
J Biomater Sci Polym Ed. 2012;23(11):1485-504. doi: 10.1163/092050611X584900. Epub 2012 May 8.
In this work, nitrogen, oxygen and air glow discharges powered by 50 Hz AC power supply are used for the treatment of type-A gelatin film cross-linked by a dehydrothermal (DHT) process. The properties of cross-linked gelatin were characterized by contact angle measurement, atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) analysis. The results showed that the water contact angle of gelatin films decrease with increasing plasma treatment time. The treatment of nitrogen, oxygen and air plasma up to 30 s had no effects on the surface roughness of the gelatin film as revealed by AFM results. The XPS analysis showed that the N-containing functional groups generated by nitrogen and air plasma, and O-containing functional groups generated by oxygen and air plasmas were incorporated onto the film surface, the functional groups were found to increase with increasing treatment time. An in vitro test using rat bone-marrow-mesenchym-derived stem cells (MSCs) revealed that the number of cells attached on plasma-treated gelatin films was significantly increased compared to untreated samples. The best enhancement of cell attachment was noticed when the film was treated with nitrogen plasma for 15-30 s, oxygen plasma for 3 s, and air plasma for 9 s. In addition, among the three types of plasmas used, nitrogen plasma treatment gave the best MSCs attachment on the gelatin surface. The results suggest that a type-A gelatin film with water contact angle of 27-28° and an O/N ratio of 1.4 is most suitable for MSCs attachment.
在这项工作中，使用由50 Hz交流电源供电的氮气、氧气和空气辉光放电来处理通过脱水热（DHT）工艺交联的A型明胶膜。通过接触角测量、原子力显微镜（AFM）和X射线光电子能谱（XPS）分析对交联明胶的性能进行了表征。结果表明，明胶膜的水接触角随着等离子体处理时间的增加而减小。AFM结果显示，氮气、氧气和空气等离子体处理30 s对明胶膜的表面粗糙度没有影响。XPS分析表明，氮气和空气等离子体产生的含氮官能团以及氧气和空气等离子体产生的含氧官能团被引入到膜表面，发现这些官能团随着处理时间的增加而增加。使用大鼠骨髓间充质干细胞（MSCs）进行的体外测试表明，与未处理的样品相比，附着在经等离子体处理的明胶膜上的细胞数量显著增加。当膜用氮气等离子体处理15 - 30 s、氧气等离子体处理3 s和空气等离子体处理9 s时，细胞附着的增强效果最佳。此外，在所使用的三种等离子体中，氮气等离子体处理在明胶表面上使MSCs附着效果最佳。结果表明，水接触角为27 - 28°且O/N比为1.4的A型明胶膜最适合MSCs附着。
