Chemical Engineering Program, Department of Industrial Engineering, Faculty of Engineering, Naresuan University, Phitsanulok 65000, Thailand.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):4475-9. doi: 10.1016/j.msec.2013.05.057. Epub 2013 Jun 6.
The attachment and growth behavior of mouse fibroblast (L929) and rat bone marrow-derived mesenchymal stem cell (MSC) on nitrogen plasma-treated and untreated gelatin films was investigated and compared. The gelatin films were prepared by solution casting (0.05% w/v) and crosslinked using dehydrothermal treatment. The crosslinked gelatin films were treated with nitrogen alternating current (AC) 50 Hz plasma systems at various treatment time. The results on the attachment and growth of two cells; L929 and MSC, on plasma-treated gelatin film showed that the number of attached and proliferated cells on plasma-treated gelatin films was significantly increased compared to untreated samples. However, no significant difference between the number of attached L929 and MSC on plasma-treated gelatin was observed. The shorter population doubling time and higher growth rate of cells cultured on plasma-treated film indicated the greater growth of cells, compared to ones on untreated films. The greatest enhancement of cell attachment and growth were noticed when the film was treated with nitrogen plasma for 9 to 15s. This suggested that the greater attachment and growth of both cells on gelatin films resulted from the change of surface properties, i.e. hydrophilicity, surface energy, and chemistry. The suitable water contact angle and oxygen/nitrogen ratio (O/N) of gelatin film for best L929 and MSC attachment were observed at 27-32° and 1.4, respectively. These conditions also provided the best proliferation of cells on plasma-treated gelatin films.
研究并比较了小鼠成纤维细胞（L929）和大鼠骨髓间充质干细胞（MSC）在氮等离子体处理和未处理明胶薄膜上的附着和生长行为。明胶薄膜通过溶液浇铸（0.05％w/v）和热脱水交联制备。用氮交流（AC）50Hz 等离子体系统对交联明胶薄膜进行不同处理时间的处理。在等离子体处理明胶薄膜上两种细胞（L929 和 MSC）附着和生长的结果表明，与未处理样品相比，附着和增殖细胞的数量明显增加。然而，在等离子体处理的明胶上附着的 L929 和 MSC 的数量没有明显差异。在等离子体处理的薄膜上培养的细胞的群体倍增时间更短，生长速率更高，表明与未处理的薄膜相比，细胞生长更快。当薄膜用氮气等离子体处理 9 到 15 秒时，观察到细胞附着和生长的最大增强。这表明，由于表面性质（即亲水性、表面能和化学性质）的变化，两种细胞在明胶薄膜上的附着和生长都得到了增强。观察到明胶薄膜的最佳水接触角和氧/氮比（O/N）分别为 27-32°和 1.4，这有利于 L929 和 MSC 的最佳附着。这些条件也为细胞在等离子体处理的明胶薄膜上的最佳增殖提供了条件。
