一种快速商业检测试剂Enterocheck WB(®)与自动血培养法在伤寒热诊断中的比较。
Comparison of a rapid commercial test, Enterocheck WB(®), with automated blood culture for diagnosis of typhoid fever.
作者信息
Anusha R, Ganesh R, Lalitha J
机构信息
Department of Paediatrics, Kanchi Kamakoti CHILDS Trust Hospital, The CHILDS Trust Medical Research Foundation, Chennai, India.
出版信息
Ann Trop Paediatr. 2011;31(3):231-4. doi: 10.1179/1465328111Y.0000000030.
BACKGROUND
Rapid diagnosis of typhoid fever is very important in the timely initiation of therapy.
OBJECTIVES
To evaluate the sensitivity and specificity of the Enterocheck WB(®) test.
METHODS
In a prospective study, the sensitivity and specificity of Enterocheck WB(®) that detects IgM antibodies to Salmonella typhi were compared with blood culture using BacT/Alert.
RESULTS
Four hundred and fifty patients with suspected typhoid fever were assessed both by blood culture and Enterocheck WB(®); 100 of them were either Enterocheck WB(®)-positive or blood culture-positive or both for S. typhi. Both tests were positive in 47 of the 100 patients. In 45 patients, Enterocheck WB(®) was positive but blood culture was negative. In eight cases, Enterocheck was negative but blood culture was positive. Using blood culture as the gold standard, the sensitivity of Enterocheck WB(®) was 85.5%, specificity 88.6%, negative predictive value 97.7% and positive predictive value 51.1%.
CONCLUSION
The Enterocheck WB(®) test can be performed at the bedside in 15 minutes and is a useful rapid method for diagnosis of typhoid fever.
背景
伤寒热的快速诊断对于及时开始治疗非常重要。
目的
评估Enterocheck WB（®）检测的敏感性和特异性。
方法
在一项前瞻性研究中，将检测伤寒沙门菌IgM抗体的Enterocheck WB（®）的敏感性和特异性与使用BacT/Alert进行的血培养进行比较。
结果
450例疑似伤寒热患者同时接受了血培养和Enterocheck WB（®）检测；其中100例患者的Enterocheck WB（®）检测、血培养检测或两者检测均呈伤寒沙门菌阳性。100例患者中有47例两种检测均为阳性。45例患者Enterocheck WB（®）检测呈阳性但血培养检测呈阴性。8例患者Enterocheck检测呈阴性但血培养检测呈阳性。以血培养为金标准，Enterocheck WB（®）的敏感性为85.5%，特异性为88.6%，阴性预测值为97.7%，阳性预测值为51.1%。
结论
Enterocheck WB（®）检测可在床边15分钟内完成，是诊断伤寒热的一种有用的快速方法。
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