Pastoor Rob, Hatta Mochammad, Abdoel Theresia H, Smits Henk L
KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Amsterdam, The Netherlands.
Diagn Microbiol Infect Dis. 2008 Jun;61(2):129-34. doi: 10.1016/j.diagmicrobio.2007.12.014. Epub 2008 Feb 13.
We developed a point-of-care test for the serodiagnosis of typhoid fever in the format of an immunochromatographic lateral flow assay. The flow assay for typhoid fever is based on the detection of Salmonella enterica serotype Typhi lipopolysaccharide-specific immunoglobulin M (IgM) antibodies. The assay was evaluated on serum samples collected in a hospital in South Sulawesi, Indonesia, where typhoid fever is endemic, and the results were compared with culture and Widal test. The sensitivity of this typhoid fever IgM flow assay for samples collected at 1st diagnosis from patients with culture-confirmed typhoid fever was determined to be 59.3%. The sensitivity ranged from 41.2% to 89.5%, depending on the duration of illness. A specificity of 97.8% was calculated based on results obtained for patients with clinical suspicion of typhoid fever that was later excluded. The assay is ideal for use as a point-of-care test in health care centers that lack the expertise and facilities to perform culture or the less specific Widal test. Because of its simplicity, the assay may also be used as a field test in remote areas.
我们开发了一种即时检验方法，以免疫层析侧向流动分析法的形式用于伤寒热的血清学诊断。伤寒热流动分析法基于检测肠炎沙门氏菌伤寒血清型脂多糖特异性免疫球蛋白M（IgM）抗体。该检测方法在印度尼西亚南苏拉威西省一家医院收集的血清样本上进行了评估，该地区伤寒热为地方病，检测结果与培养法和维达试验进行了比较。这种伤寒热IgM流动分析法对培养确诊的伤寒热患者首次诊断时采集的样本的敏感性为59.3%。根据病程不同，敏感性范围为41.2%至89.5%。根据对临床怀疑为伤寒热但后来排除的患者的检测结果计算得出特异性为97.8%。该检测方法非常适合在缺乏进行培养或特异性较低的维达试验的专业知识和设施的医疗保健中心用作即时检验。由于其操作简单，该检测方法也可在偏远地区用作现场检测。
