Qin Xin-cheng, Tian Jun-hua, Wang Jian-bo, Lu Xin, Sun Qiang-zheng, Jin Dong, Zhou Dun-jin, Xu Jian-guo, Zhang Yong-zhen
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2011 Jun;32(6):608-12.
To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicornis and Rhipicephalus microplus.
Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0.
The ticks belonged to Haemaphysalis longicornis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results.
The method based on morphology that combined with molecular marker seemed a good method for the identification of ticks.
建立一种结合形态学和分子标记的方法来鉴定长角血蜱和微小牛蜱。
在湖北和河南发热伴血小板减少综合征流行区的家畜和野外环境中采集蜱虫。在通过PCR扩增蜱虫的12S rDNA并随后进行测序之前，根据形态特征对蜱虫进行分类。使用PAUP4.0构建系统发育树。
通过对蜱虫形态特征的观察和分析，确定蜱虫属于长角血蜱和微小牛蜱。克隆并测序了12S rDNA，数据证实了形态学鉴定结果。
基于形态学结合分子标记的方法似乎是一种鉴定蜱虫的好方法。
