微小牛蜱、长角血蜱和血红扇头蜱肌动蛋白编码cDNA的分子克隆及序列分析
Molecular cloning and sequence analysis of cDNAs encoding for Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus actins.
作者信息
da Silva Vaz Itabajara, Imamura Saiki, Nakajima Chie, de Cardoso Felipe Cardoso, Ferreira Carlos Alexandre Sanchez, Renard Gaby, Masuda Aoi, Ohashi Kazuhiko, Onuma Misao
机构信息
Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Sapporo 060-0818, Hokkaido, Japan.
出版信息
Vet Parasitol. 2005 Jan 20;127(2):147-55. doi: 10.1016/j.vetpar.2004.10.002. Epub 2004 Nov 21.
The nucleotide and deduced amino acid sequences of the actins from ticks, Boophilus microplus, Haemaphysalis longicornis and Rhipicephalus appendiculatus, have been determined. Nucleotide sequence analysis showed open reading frames of 1128-nucleotide-long encoding proteins of 376 amino acids with a predicted molecular weight of 41.82 kDa each. Comparison between the nucleic acid and deduced amino acid sequences as well as structural and phylogenetic analyses of these genes confirmed the high similarity among actins from ticks in comparison to other species.
已测定微小牛蜱、长角血蜱和血红扇头蜱肌动蛋白的核苷酸序列及推导的氨基酸序列。核苷酸序列分析显示，开放阅读框长度为1128个核苷酸，编码376个氨基酸的蛋白质，预计每个蛋白质的分子量为41.82 kDa。这些基因的核酸序列与推导的氨基酸序列之间的比较以及结构和系统发育分析证实，蜱类肌动蛋白与其他物种相比具有高度相似性。
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