Mechanisms of Drug Toxicity Group, Department of Pharmaceutical Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
Environ Toxicol Pharmacol. 1996 Apr 15;1(2):97-102. doi: 10.1016/1382-6689(95)00013-5.
Methaemoglobin generation by monoacetyl dapsone hydroxylamine in non-diabetic and diabetic erythrocytes was investigated in vitro. Methaemoglobin formation in purified haemoglobin isolated from both types of erythrocytes as well as haemolysates from both diabetic and non-diabetic erythrocytes did not differ. Prior to 18 h incubation with 10 and 20 mM glucose diabetic erythrocytes were significantly less sensitive to monoacetyl dapsone-induced methaemoglobinaemia. After pre-incubation the differential was lost although significant change in glutathione concentrations could not be shown between the two cell types. NADH-diaphorase levels measured in diabetics and non-diabetics did not significantly differ. It is possible that diabetic cells display reduced hydroxylamine-mediated methaemoglobin generation due to differences in glutathione metabolism.
我们研究了单乙酰对氨苯砜羟胺在非糖尿病和糖尿病红细胞中生成高铁血红蛋白的情况。从两种类型的红细胞中分离的血红蛋白以及来自糖尿病和非糖尿病红细胞的溶血产物中的高铁血红蛋白形成没有差异。在与 10 和 20 mM 葡萄糖孵育 18 小时之前，糖尿病红细胞对单乙酰对氨苯砜诱导的高铁血红蛋白血症的敏感性显著降低。预孵育后，这种差异消失了，尽管两种细胞类型之间的谷胱甘肽浓度没有显著变化。在糖尿病患者和非糖尿病患者中测量的 NADH-黄递酶水平没有显著差异。由于谷胱甘肽代谢的差异，糖尿病细胞可能显示出高铁血红蛋白生成减少。
