Mechanisms of Drug Toxicity Group, Department of Pharmaceutical Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.
Environ Toxicol Pharmacol. 1998 Jan;5(1):61-7. doi: 10.1016/s1382-6689(97)10006-0.
Nitrite, monoacetyl dapsone hydroxylamine, 4-aminophenol and disulfiram-mediated methaemoglobin formation was studied in human diabetic and non-diabetic erythrocytes in vitro. Diabetic intact erythrocytes were significantly less sensitive compared with those of non-diabetics to haemoglobin oxidation caused by the hydroxylamine, nitrite and 4-aminophenol, but not disulfiram. In haemolysates, differential sensitivity did occur with disulfiram and was partially retained with 4-aminophenol and nitrite. The differences were lost with 4-aminophenol, nitrite and disulfiram in the presence of haemoglobin purified from the respective erythrocyte types. Diethyl maleate reduced methaemoglobin formation in non-diabetic intact erythrocytes with 4-aminophenol, the hydroxylamine and disulfiram, but not with nitrite. Overall, the differential sensitivity to methaemoglobin formation seen in diabetic compared with non-diabetic erythrocytes, is probably linked to differences in the respective cells' cytosolic anti-oxidant systems.
亚硝酸钠、单乙酰对氨苯砜羟胺、对氨基酚和双硫仑介导的正铁血红蛋白形成在体外的糖尿病患者和非糖尿病患者的人红细胞中进行了研究。与非糖尿病患者的红细胞相比，糖尿病完整红细胞对羟胺、亚硝酸钠和对氨基酚引起的血红蛋白氧化的敏感性明显降低，但对双硫仑的敏感性没有降低。在红细胞溶血液中，与双硫仑和部分保留的对氨基酚和亚硝酸钠发生差异敏感性。在从各自红细胞类型中纯化的血红蛋白存在的情况下，差异消失了，与对氨基酚、亚硝酸钠和双硫仑。马来酸二乙酯降低了非糖尿病完整红细胞中与对氨基酚、羟胺和双硫仑形成的正铁血红蛋白，但不降低亚硝酸钠。总的来说，与非糖尿病红细胞相比，糖尿病红细胞中观察到的正铁血红蛋白形成的差异敏感性可能与各自细胞的细胞溶质抗氧化系统的差异有关。
