Gu G H, Lim C K
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
J Chromatogr. 1990 Aug 31;515:183-92. doi: 10.1016/s0021-9673(01)89312-2.
The separation of small, ionizable compounds of biomedical interest on porous graphitic carbon is described. The retention of anionic compounds is dominated by electronic interaction between the solute and the delocalized electron clouds on the graphitized carbon, while cationic compounds are mainly retained by reversed-phase interaction with the hydrophobic carbon surface. Anionic and cationic compounds can be separated simultaneously with a mobile phase containing an electronic modifier (e.g., trifluoroacetic acid) and an organic modifier (e.g., acetonitrile) for elution. Examples of applications include the measurement of oxalic acid in urine, the determination of creatine and creatinine in urine and in serum, the separation of basic drugs (remoxipride and FLA 981) and the simultaneous analysis of pertechnetate anion and the cationic technetium-amine complexes.
本文描述了在多孔石墨化碳上分离具有生物医学意义的可电离小分子化合物的方法。阴离子化合物的保留主要由溶质与石墨化碳上离域电子云之间的电子相互作用主导，而阳离子化合物主要通过与疏水性碳表面的反相相互作用保留。阴离子和阳离子化合物可以在含有电子改性剂（如三氟乙酸）和有机改性剂（如乙腈）的流动相洗脱下同时分离。应用实例包括尿液中草酸的测定、尿液和血清中肌酸和肌酐的测定、碱性药物（瑞莫必利和FLA 981）的分离以及高锝酸盐阴离子和阳离子锝-胺络合物的同时分析。
