Lim C K
Division of Clinical Cell Biology, MRC Clinical Research Centre, Middlesex, UK.
Biomed Chromatogr. 1989 Mar;3(2):92-3. doi: 10.1002/bmc.1130030212.
The oxo anions of technetium-99m and rhenium, pertechnetate (TcO4-) and perrhenate (ReO4-), have been separated by high performance liquid chromatography on porous graphitic carbon with aqueous trifluoroacetic acid or salt solutions as eluent. Chromatographic retention was a result of electronic interaction between the lone pair electrons of the anions and the delocalised pi-electron clouds of the porous graphitic carbon. Retention and resolution can be controlled by the concentration and/or species of eluents which can compete with the solutes for electronic interaction being used for elution.
锝-99m和铼的含氧阴离子，即高锝酸盐（TcO4-）和高铼酸盐（ReO4-），已通过高效液相色谱法在多孔石墨碳上进行分离，以三氟乙酸水溶液或盐溶液作为洗脱剂。色谱保留是阴离子的孤对电子与多孔石墨碳的离域π电子云之间电子相互作用的结果。保留和分离度可通过洗脱剂的浓度和/或种类来控制，这些洗脱剂可与溶质竞争用于洗脱的电子相互作用。
