Haginaka J, Wakai J, Yasuda N, Yasuda H, Kimura Y
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
J Chromatogr. 1990 Aug 31;515:59-66. doi: 10.1016/s0021-9673(01)89302-x.
Internal-surface reversed-phase (ISRP) silica supports having N-octanoylaminopropyl phases bound to the internal surfaces of the porous silica and N-(2,3-dihydroxypropyl)aminopropyl phases bound to the external surfaces were synthesized from silica particles differing in nominal pore diameters and specific surface areas. These ISRP supports were characterized with regard to physical and chromatographic properties. The support with an N-octanoylaminopropyl phase coverage of 485 mumol/g and an average pore diameter of 65 A was the most suitable for the direct-injection determination of hydrophilic or hydrophobic drugs in serum or plasma. Non-steroidal anti-inflammatory (acetylsalicylic acid and salicylic acid) and tricyclic antidepressant drugs (desipramine and nortriptyline) in serum were successfully determined with this support and an acidic eluent.
通过具有不同标称孔径和比表面积的二氧化硅颗粒，合成了内表面反相（ISRP）硅胶载体，其在内表面结合了N - 辛酰氨基丙基相，在外表面结合了N - （2,3 - 二羟丙基）氨基丙基相。对这些ISRP载体的物理和色谱性质进行了表征。N - 辛酰氨基丙基相覆盖率为485 μmol/g且平均孔径为65 Å的载体最适合直接进样测定血清或血浆中的亲水性或疏水性药物。使用该载体和酸性洗脱液成功测定了血清中的非甾体抗炎药（乙酰水杨酸和水杨酸）和三环类抗抑郁药（地昔帕明和去甲替林）。
