Department of Environmental Toxicology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, Japan.
Environ Toxicol Pharmacol. 1996 Dec 20;2(4):373-80. doi: 10.1016/s1382-6689(96)00074-9.
The effects of tributyltin on the expression of immediate early genes, c-fos and c-jun, were examined in PC12 cells using reverse transcription polymerase chain reaction analysis. Tributyltin at concentrations of more than 0.4 μM induced the expression of c-fos after 15 min exposure. The induction of c-fos was accompanied with c-jun expression. Tributyltin-induced c-fos expression was abolished completely by actinomycin D, indicating it was due to transcriptional activation of the gene. Chelation of intracellular Ca(2+) suppressed the expression of c-fos markedly, while removal of external Ca(2+) did not. These results suggest that Ca(2+) mobilized from intracellular stores played a major role in the tributyltin-induced transcriptional activation of c-fos in PC12 cells.
采用逆转录聚合酶链反应分析的方法，研究了三丁基锡对 PC12 细胞即刻早期基因 c-fos 和 c-jun 表达的影响。三丁基锡在浓度高于 0.4μM 时，可在 15 分钟暴露后诱导 c-fos 的表达。c-fos 的诱导伴随着 c-jun 的表达。三丁基锡诱导的 c-fos 表达完全被放线菌素 D 所阻断，表明这是由于基因的转录激活。螯合细胞内 Ca(2+)可显著抑制 c-fos 的表达，而去除细胞外 Ca(2+)则无此作用。这些结果表明，细胞内库中动员的 Ca(2+)在三丁基锡诱导 PC12 细胞 c-fos 的转录激活中起主要作用。
