从 Zn/Cd 超积累生态型和非超积累生态型的Sedum alfredii Hance 中分离出的金属耐受蛋白的功能分析。
Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non-hyperaccumulating ecotype of Sedum alfredii Hance.
机构信息
MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
出版信息
FEBS Lett. 2011 Aug 19;585(16):2604-9. doi: 10.1016/j.febslet.2011.07.013. Epub 2011 Jul 20.
The Zn/Cd hyperaccumulating ecotype (HE) of Sedum alfredii Hance can accumulate 24- and 28-fold higher leaf and stem Zn concentrations when compared with the non-hyperaccumulating ecotype (NHE) of Sedum. Heterologous expression of a metal tolerance protein (MTP1) encoding gene from HE plants (SaMTP1) or the homologous gene from NHE plants (SnMTP1) suppressed Zn(2+) hypersensitivity in the Δzrc1 yeast mutant. In plants, SaMTP1 localized to the tonoplast. Furthermore, MTP1 transcript level in the shoot of HE plants was more than 80-fold higher than that of NHE plants. The transcript level of SaMTP1 in shoot was up-regulated 1-fold by Zn(2+) while the expression of SnMTP1 was slightly inhibited. These data suggest that SaMTP1 can play an important role in Zn accumulation in HE plants.
超积累型（HE）凹叶景天（Sedum alfredii Hance）的叶片和茎中锌浓度比非超积累型（NHE）高出 24-28 倍。来自 HE 植物的金属耐受蛋白（MTP1）编码基因（SaMTP1）或来自 NHE 植物的同源基因（SnMTP1）的异源表达抑制了Δzrc1 酵母突变体的锌（2+）超敏反应。在植物中，SaMTP1定位于液泡膜上。此外，HE 植物地上部分的 MTP1 转录本水平比 NHE 植物高 80 多倍。锌（2+）处理使 HE 植物地上部分的 SaMTP1 转录本水平上调 1 倍，而 SnMTP1 的表达则略有抑制。这些数据表明，SaMTP1 可能在 HE 植物的锌积累中发挥重要作用。
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