MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
FEBS Lett. 2011 Aug 19;585(16):2604-9. doi: 10.1016/j.febslet.2011.07.013. Epub 2011 Jul 20.
The Zn/Cd hyperaccumulating ecotype (HE) of Sedum alfredii Hance can accumulate 24- and 28-fold higher leaf and stem Zn concentrations when compared with the non-hyperaccumulating ecotype (NHE) of Sedum. Heterologous expression of a metal tolerance protein (MTP1) encoding gene from HE plants (SaMTP1) or the homologous gene from NHE plants (SnMTP1) suppressed Zn(2+) hypersensitivity in the Δzrc1 yeast mutant. In plants, SaMTP1 localized to the tonoplast. Furthermore, MTP1 transcript level in the shoot of HE plants was more than 80-fold higher than that of NHE plants. The transcript level of SaMTP1 in shoot was up-regulated 1-fold by Zn(2+) while the expression of SnMTP1 was slightly inhibited. These data suggest that SaMTP1 can play an important role in Zn accumulation in HE plants.
超积累型（HE）凹叶景天（Sedum alfredii Hance）的叶片和茎中锌浓度比非超积累型（NHE）高出 24-28 倍。来自 HE 植物的金属耐受蛋白（MTP1）编码基因（SaMTP1）或来自 NHE 植物的同源基因（SnMTP1）的异源表达抑制了Δzrc1 酵母突变体的锌（2+）超敏反应。在植物中，SaMTP1定位于液泡膜上。此外，HE 植物地上部分的 MTP1 转录本水平比 NHE 植物高 80 多倍。锌（2+）处理使 HE 植物地上部分的 SaMTP1 转录本水平上调 1 倍，而 SnMTP1 的表达则略有抑制。这些数据表明，SaMTP1 可能在 HE 植物的锌积累中发挥重要作用。
