Ge Jun, Lin Jiayu, Wu Zhiying, Xu Kuan, Tao Jingyu, Lin Haizhong, Tian Shengke, Lu Lingli
Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Science, Zhejiang University, Hangzhou 310058, China.
Agricultural Technology Extension Center of Huangyan, Taizhou 318020, China.
Life (Basel). 2022 May 23;12(5):768. doi: 10.3390/life12050768.
Zn pollution is a potential toxicant for agriculture and the environment. is a Zn/Cd hyperaccumulator found in China and has been proven as a useful resource for the phytoremediation of Zn-contaminated sites. However, the molecular mechanism of Zn uptake in is limited. In this study, the function of SaPCR2 on Zn uptake in was identified by gene expression analysis, yeast function assays, Zn accumulation and root morphology analysis in transgenic lines to further elucidate the mechanisms of uptake and translocation of Zn in . The results showed that was highly expressed in the root elongation zone of the hyperaccumulating ecotype (HE) , and high Zn exposure downregulated the expression of in the HE root. The heterologous expression of in yeast suggested that SaPCR2 was responsible for Zn influx. The overexpression of in the non-hyperaccumulating ecotype (NHE) significantly increased the root uptake of Zn, but did not influence Mn, Cu or Fe. SR-μ-XRF technology showed that more Zn was distributed in the vascular buddle tissues, as well as in the cortex and epidermis in the transgenic lines. Root morphology was also altered after overexpression, and a severe inhibition was observed. In the transgenic lines, the meristematic and elongation zones of the root were lower compared to the WT, and Zn accumulation in meristem cells was also reduced. These results indicate that SaPCR2 is responsible for Zn uptake, and mainly functions in the root elongation zone. This research on could provide a theoretical basis for the use of genetic engineering technology in the modification of crops for their safe production and biological enhancement.
锌污染对农业和环境来说是一种潜在的有毒物质。[植物名称]是在中国发现的一种锌/镉超积累植物，已被证明是用于锌污染场地植物修复的一种有用资源。然而，[植物名称]吸收锌的分子机制尚不清楚。在本研究中，通过基因表达分析、酵母功能测定、转基因株系中的锌积累和根系形态分析，确定了SaPCR2在[植物名称]吸收锌过程中的功能，以进一步阐明[植物名称]吸收和转运锌的机制。结果表明，[植物名称]在超积累生态型（HE）[植物名称]的根伸长区高度表达，高锌暴露下调了HE[植物名称]根中[该基因]的表达。[该基因]在酵母中的异源表达表明SaPCR2负责锌的流入。在非超积累生态型（NHE）[植物名称]中过表达[该基因]显著增加了根对锌的吸收，但不影响锰、铜或铁。SR-μ-XRF技术表明，转基因株系中更多的锌分布在维管束组织以及皮层和表皮中。过表达[该基因]后根系形态也发生了改变，并观察到严重的抑制作用。在转基因株系中，根的分生区和伸长区比野生型低，分生细胞中的锌积累也减少。这些结果表明，SaPCR2负责锌的吸收，主要在根伸长区发挥作用。对[植物名称]的这项研究可为利用基因工程技术改良作物以实现其安全生产和生物强化提供理论依据。
