Yoneyama T, Takeuchi K, Watanabe Y, Harada H, Ohbayashi A, Tanaka Y, Yuasa T, Saito I, Miyamura T
Department of Enteroviruses, National Institute of Health, Tokyo.
Jpn J Med Sci Biol. 1990 Jun;43(3):89-94. doi: 10.7883/yoken1952.43.89.
We found the presence of hepatitis C virus (HCV) infection in liver tissues of hepatocellular carcinoma (HCC) patients who had antibodies to HCV but no serological markers for hepatitis B virus infection by the sensitive reverse transcription/polymerase chain reaction (R/PCR) method. The primers used were derived from the non-structural (NS) 3 and/or the structural (C/E) region. Amplified cDNA sequences of HCV were detected in either cancerous or non-cancerous portion of liver tissues from four out of eight HCC patients with primers of NS3 region. Similar but less efficient results were obtained with primers of C/E region. These results indicate that HCV persists in the liver tissue of HCC. A possible role of persistent infection of HCV for the development of HCC is discussed.
我们通过敏感的逆转录/聚合酶链反应（R/PCR）方法，在肝细胞癌（HCC）患者的肝组织中发现了丙型肝炎病毒（HCV）感染，这些患者具有抗HCV抗体，但没有乙型肝炎病毒感染的血清学标志物。所用引物来自非结构（NS）3区和/或结构（C/E）区。在8例HCC患者中的4例，使用NS3区引物在肝组织的癌组织或非癌组织中检测到HCV扩增的cDNA序列。使用C/E区引物也获得了类似但效率较低的结果。这些结果表明HCV在HCC肝组织中持续存在。本文讨论了HCV持续感染在HCC发生发展中的可能作用。
