Department of Veterinary Paramedical Science, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan; United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Environ Toxicol Pharmacol. 2002 Aug;12(1):55-8. doi: 10.1016/s1382-6689(02)00005-4.
Gel filtration chromatography was performed on cytosol preparation of hen spinal cord to find molecular target(s) for organophosphorus-induced delayed neurotoxicity (OPIDN). Three binding peaks of [(3)H]diisopropyl phosphorofluoridate (DFP), an organophosphate that induces OPIDN, were separated from the cytosol preparation. The activities of acetylcholinesterase (AChE) and neuropathy target esterase (NTE) that has been proposed as a screening method for OPIDN eluted in the fractions within these two DFP binding peaks. However, the other peak had none of the activities of AChE and NTE. Therefore, this DFP binding proteins in cytosol may be peculiar to the pathogenesis of OPIDN.
采用凝胶过滤色谱法对鸡脊髓胞质液进行分析，以寻找有机磷诱导迟发性神经病（OPIDN）的分子靶标。从胞质液中分离出有机磷杀虫剂二异丙基氟磷酸（DFP）与三个[（3）H]DFP 结合峰，该有机磷可诱导 OPIDN。已提出乙酰胆碱酯酶（AChE）和神经毒性靶酯酶（NTE）的活性作为 OPIDN 的筛选方法，在这两个 DFP 结合峰的部分中洗脱。然而，其他峰则没有 AChE 和 NTE 的活性。因此，胞质液中的这种 DFP 结合蛋白可能是 OPIDN 发病机制所特有的。
