Department of Biology, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong, PR China.
Environ Toxicol Pharmacol. 2003 Sep;14(3):109-16. doi: 10.1016/S1382-6689(03)00045-0.
This research investigates the protective effect of N-acetylcysteine (NAC) against carbon tetrachloride (CCl(4))- and trichloroethylene (TCE)-induced hepatotoxicity in rats. A single dose of 1.25 ml/kg of 20% CCl(4) in corn oil, administered orally, or 20% TCE, administered intraperitoneally, produced significantly elevated levels of serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT) activities. Histopathological examinations showed massive centrilobular necrosis and fat accumulation in CCl(4)-treated animals. In the curative test, especially in animals treated with higher dosages of NAC, there was significant reduction in SGPT and SGOT levels. Although there was no sign of abnormality in the livers of rats treated with TCE, NAC demonstrated its action against TCE-induced elevation of transaminases in the enzyme assays. Compared to the curative tests, the overall performance of NAC against toxin-induced toxicity in the preventive tests was poor. Even at the highest dosage applied, the effect was not as prominent as that achieved in the curative test. It is therefore concluded that NAC is effective for lowering chemical-induced elevated levels of SGPT and SGOT in the curative mode.
本研究旨在探讨 N-乙酰半胱氨酸 (NAC) 对四氯化碳 (CCl(4)) 和三氯乙烯 (TCE) 诱导的大鼠肝毒性的保护作用。给予大鼠口服 1.25 毫升/千克 20% CCl(4) 玉米油溶液或腹腔内注射 20% TCE，可显著升高血清谷氨酸丙酮酸转氨酶 (SGPT) 和血清谷氨酸草酰乙酸转氨酶 (SGOT) 活性。组织病理学检查显示 CCl(4) 处理的动物出现大量中央小叶坏死和脂肪堆积。在治疗试验中，尤其是在接受较高剂量 NAC 治疗的动物中，SGPT 和 SGOT 水平显著降低。虽然 TCE 处理的大鼠肝脏没有异常迹象，但 NAC 在酶测定中显示出对 TCE 诱导的转氨酶升高的作用。与治疗试验相比，NAC 在预防试验中对毒素诱导的毒性的总体表现较差。即使应用最高剂量，其效果也不如治疗试验中明显。因此，结论是 NAC 在治疗模式下可有效降低化学诱导的 SGPT 和 SGOT 升高。
