Yoshihara T, Igarashi M, Fermin C D
Department of Otorhinolaryngology, Tokyo Women's Medical College, Japan.
Acta Otolaryngol. 1990 Nov-Dec;110(5-6):366-73. doi: 10.3109/00016489009107456.
We studied the morphological changes and the precise localization of Na+, K(+)-ATPase activity in the developing chick tegmentum vasculosum (TV) with a cytochemical method utilizing K(+)-p-nitrophenylphosphatase (K(+)-NPPase). At stage 37, epithelial folds (lamellae) were relatively well developed, and the light and the dark cells were clearly identified. At stage 41 to 42, Na+, K(+)-ATPase activity appeared in the folded cytoplasmic processes of the dark cells which interdigitated with those of the light cells. The present results indicate that the TV is involved in the production of the cochlear endolymph prenatally (at least up to stage 41), and that the dark cell plays a central role in the maintenance of the ionic composition of the endolymph.
我们利用对硝基苯磷酸钾（K(+)-NPPase）的细胞化学方法，研究了发育中的鸡顶盖血管层（TV）的形态变化以及Na+、K(+)-ATP酶活性的精确定位。在第37阶段，上皮褶皱（薄片）发育相对良好，亮细胞和暗细胞清晰可辨。在第41至42阶段，Na+、K(+)-ATP酶活性出现在与亮细胞的指状交错的暗细胞的折叠细胞质突起中。目前的结果表明，TV在产前（至少到第41阶段）参与了耳蜗内淋巴的产生，并且暗细胞在内淋巴离子组成的维持中起核心作用。
