Department of Chemistry, Chung Yuan Christian University, Chung-Li, Taoyuan County 32023, Taiwan, ROC.
Biomaterials. 2011 Oct;32(30):7347-54. doi: 10.1016/j.biomaterials.2011.06.069. Epub 2011 Jul 23.
The three-dimensional (3D) biochips prepared in this study are composed of a glass microscopy slide arrayed with amino aerogel dots. The amino aerogel was produced using the sol-gel process, with an ionic liquid as the template followed by a solvent extraction to remove the template and build a three-dimensional mesoporous structure. The FTIR spectrum verified that the major template was removed and the (29)Si solid-state NMR spectra recognized the cross-linkages in the SiO(2) network structure. SEM images measured the particles at around 100 nm. After grinding, the BET analysis confirmed that the nano-size amino aerogel powders had exhibited specific surface area of 188 m(2)/g, pore volume of 0.83 cm(3)/g, and average pore size of 16.2 nm. The as-prepared amino aerogel surface contained amino functional groups capable of performing a sandwich immunoassay. The primary antibody was immobilized on the internal surface of the arrayed amino aerogel to capture its affinity antigen. On the top of the captured antigen, the report antibody was read its labeling fluorescent dye. In comparison to the corresponding two-dimensional (2D) biochip, the 3D amino aerogel biochips were observed to amplify signal intensities more effectively due to their remarkable capturing capability.
本研究制备的三维（3D）生物芯片由排列有氨基气凝胶点的玻璃显微镜载玻片阵列组成。氨基气凝胶是通过溶胶-凝胶过程制备的，以离子液体为模板，然后通过溶剂萃取去除模板并构建三维介孔结构。FTIR 光谱证实主要模板已被去除，（29）Si 固态 NMR 光谱识别出 SiO2 网络结构中的交联。SEM 图像测量的颗粒约为 100nm。研磨后，BET 分析证实纳米级氨基气凝胶粉末的比表面积为 188m2/g，孔体积为 0.83cm3/g，平均孔径为 16.2nm。所制备的氨基气凝胶表面含有氨基官能团，能够进行三明治免疫测定。将抗体固定在排列的氨基气凝胶的内表面上，以捕获其亲和抗原。在捕获抗原的顶部，报告抗体读取其标记的荧光染料。与相应的二维（2D）生物芯片相比，由于其显著的捕获能力，3D 氨基气凝胶生物芯片被观察到能够更有效地放大信号强度。
