Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran ; Nanobiotechnology center, Department of Chemical Engineering, Jondishapour University of Technology, Dezful, Iran.
Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
Iran J Basic Med Sci. 2013 Dec;16(12):1259-65.
OBJECTIVE(S): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.
In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.
RESULTS on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.
The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes.
目的（S）：微阵列是基因组学和蛋白质组学研究的潜在分析工具，它需要合适的基质来进行生物分子的涂层和杂交。
在这项研究中，在预先涂有聚-L-赖氨酸（PLL）的载玻片上制备了氧化琼脂糖的薄膜。部分活化琼脂糖的醛基与 PLL 胺基以共价键连接；也与生物分子的氨基结合。这些键通过紫外线照射固定。将制备的基底与仅涂有琼脂糖和 PLL 的载玻片进行了比较。
原子力显微镜（AFM）的结果表明，琼脂糖提供了三维表面，其对生物分子的负载和结合能力高于具有二维表面的 PLL 涂层表面。此外，在琼脂糖-PLL 涂层基底上进行的杂交反应中的信号与噪声比分别比琼脂糖和 PLL 涂层基底提高了两倍和四倍。
在杂交中，琼脂糖-PLL 微阵列具有最高的信号（2546）和最低的背景信号（205），表明制备的载玻片适合分析宽浓度范围的分析物。
